Yes. The larynx, or voice box, is a hormone-dependent organ. Testosterone causes the vocal chords to thicken and lengthen, deepening the voice, while the cartilage of the larynx grows, further influencing the tone of voice. These changes can start to be observed anywhere from 3-6 from beginning Testosterone, and the maximum extent of these changes are often seen within 1-2 years, depending on dose.